Western Blot SDS-PAGE

Separating Protein with SDS-PAGE: Procedure and Applications

Video: Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture

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Importance of Sample Preparation and Choosing the Right Gel for SDS-PAGE - Scientific Videos | Thermo Fisher Scientific US

SOLVED: Calculate the preparation of all samples to be run on your SDS-PAGE gel. You need to load 10 μg total protein in 3 μl. You need to prepare 30 μl of this solution that has protein and 1X sample buffer (water is used for dilution). Complete the table below.Sample Protein Protein Water 5X Sample Total Sample Conc. Sample (μg) Buffer Volume (μl) (μl) (μl)CRUDE 30Desalt 30Peak Fraction 30Peak Fraction 30Note: You must complete this table before coming to class.